Gene Expression Analysis

お問い合わせ

Gene expression profiling analysis is an important part of the drug development process. It is a powerful tool that can be used to uncover the complex molecular "signature" of a disease and provide information that can determine efficacy of new therapeutic agents.

Harnessing the power of NanoString and other PCR-based technologies, Labcorp can provide a variety of gene expression analysis services (DNA and RNA) to allow researchers to gain a molecular snapshot of the tumor microenvironment and to provide answers that can assist researchers with decisions during early-stage drug discovery by confirming the expected mechanism of action and target engagement of a therapeutic. These services may also be applied to cell culture studies for proof-of-concept research prior to advancing to in vivo models.

  • NanoString

    A recent addition to Labcorp’s service portfolio, NanoString’s nCounter® Analysis System and the GeoMx® Digital Spatial Profiler (DSP) can be used to inform how a drug modulates gene expression and the signaling pathways that regulate tumor growth.

    The NanoString nCounter® allows for analysis of up to 800 RNA, DNA or protein targets simultaneously with high sensitivity and precision. The system utilizes molecular "barcodes" and single molecule imaging to quantitate hundreds of unique transcripts in a single sample.

    Other key advantages of using the NanoString nCounter® include:

    • A number of “off-the-shelf panels” for oncology (human and mouse) available, customizable to include up to 55 additional unique targets
    • Optimized for challenging sample types (FFPE, tissue, lysates and biofluids)
    • Only low amounts of precious sample material required
    • Accurate and faster than qPCR (no amplification requirements) and simpler than next generation sequencing

    While NanoString nCounter® provides great quantitative data on gene or protein expression levels from tumor samples, spatial context is key to gaining further insights into tissue biology. NanoString GeoMx® DSP is the spatial biology solution and it can be used to gain a deeper understanding of the tumor and the complex interactions within the tumor microenvironment.

    The NanoString GeoMx® DSP technology builds upon the highly robust NanoString nCounter® digital barcode technology, providing morphological context and high-plex protein or gene expression profiling from just one prepared slide sample. The technology uses software that offers tremendous flexibility when selecting a region-of-interest strategy (geometric, segmentation, cell-type specific, contour, gridded) to visualize spatial architecture and analyze multiple biomarkers of a tissue sample. The data analysis software in GeoMx® DSP supports easy visualization and interpretation of data. High-resolution images can be displayed alongside quantitative profiling data, allowing interaction with either dataset in real time.

    Labcorp is the only preclinical CRO, globally, to offer NanoString’s GeoMx® DSP technology.

    In addition to being utilized as a standalone in vitro service for genetic expression screening using cell-based models, NanoString may also be used in an ex vivo setting to analyze the drug mechanism of action in in vivo animal models, thereby offering the ability to screen multiple therapeutics to identify promising lead compounds.

  • リアルタイム PCR

    当社では、96 ウェルプレートの 4 色の読取りが可能なリアルタイム PCR システム StepOnePlus™ を導入しており、SNP ジェノタイピング、遺伝子発現解析、microRNA 発現、転座解析、遺伝子検出などのアッセイに対応しています。培養細胞、新鮮組織、凍結組織で特異遺伝子が発現しているかどうかの見極めは、当社の PCR スペシャリストにお任せください。各種細胞株、マウス種、組織、腫瘍の遺伝子発現測定であっても、さまざまな治療における異なる遺伝子の発現モニタリングであっても、お客様が最適な研究をデザインできるようお手伝いいたします。

  • 細胞株と腫瘍遺伝子の発現プロファイリング

    遺伝子発現プロファイリングでは、数千個の遺伝子の活動(発現)を一括測定し、細胞機能の全体図を作成します。RNA は次世代シーケンシングによって配列および定量化され、特異遺伝子の数千もの既知の RNA 配列に合わせて並べられます。こうしたプロファイルによって、たとえば、活発に分裂している細胞や、細胞の特定の治療に対する反応、各種細胞株、マウス種、組織、腫瘍で差次的に発現する遺伝子などが分かります。 Labcorp collaborates with a partner laboratory that specializes in sequencing the RNA.シーケンシング情報を入手した後、ソフトウェアを使って定量化、整列、データ分析を行っています。当社では、単一遺伝子、関連遺伝子のセット、経路および プロセスに特異的な遺伝子のグループ、ゲノム全体の発現をサンプル間で互いに比較し、その結果を簡単なグラフと図で表すことができます。

Access a customized offering to meet your gene expression requirements

At Labcorp, we combine state-of-the-art technologies with a customized service offering to meet your gene expression requirements. Our highly specialized team has a wealth of knowledge and experience in developing, optimizing, and validating gene expression readouts throughout the entire drug discovery process. We work closely with you to design a tailored workflow to generate a wealth of data to help you uncover the mechanism of action of your compound(s) and what signaling pathways and/or downstream genes are regulated in the context of tumor growth. We pride ourselves in having an extensive portfolio of animal/tumor models to ensure we can provide our clients with the “best fit” model for their project. Contact us today to discuss your study requirements and how our gene expression analysis services can add new insights into your research projects. 

lab-getty

Spotlight

nCounter® IO 360™ パネルおよびフローサイトメトリーは、トランスクリプトームと免疫サブセット免疫表現型プロファイルを組み合わせ、腫瘍の免疫療法に対する包括的な分析をもたらします。

Transcriptomic profiling can be used to examine the interaction between the tumor microenvironment (TME) and the immune system and is a powerful tool to investigate the mechanistic action of immunotherapies. In this Tech Spotlight, we demonstrate how gene expression measured in solid tumors using the nCounter® PanCancer IO 360™ panel (NanoString Technologies) can be combined with flow cytometry to provide a comprehensive evaluation of the effects that immunotherapy has on the anti-tumor immune response.

お問い合わせはこちら

お問い合わせ