Cell Culture

Labcorp cell culture analysts have experience with over 500 cell lines, including models for over 75 histotypes, covering acute monocytic leukemia (AML) to vulva leiomyosarcoma. We have an experienced team of analysts who are familiar with various mechanisms for cell culture scale up including culture in plates, flasks, roller bottles, spinner flasks, and multi-layered flasks. Importantly, we have capacity to process thousands of flasks per week to support large- and small-scale animal work including support for over 40 models on our FastTrack program, which includes initiation of cell culture within 2 weeks of study signing.

Labcorp can monitor the cell morphology of your cells in culture using an Olympus CKX53F inverted fluorescence microscope with phase contrast and a 1.4MP CCD camera. 治療の前後や治療中に、長期にわたって、あるいは移植前の特定のタイミングでも画像をチェックし、細胞が正常に成長していることを確認しましょう。

We o   can monitor the growth of your cells over time and compare different cell variants, cells grown in different media, or cells grown with different treatments. We have two Luna CellDrop Automated Cell Counters that can accurately distinguish the live from the dead cells using trypan blue or AO/PI and count each population. Our cell culture team has a vast amount of experience culturing multiple types of cells including immortalized tumor cells, primary cells, immune cells, neurospheres, and spheroidstumor cells, primary cells, and neurospheres. The team also has experience performing colony formation assays to detect the tumorigenic potential of transformed cells and the tumor suppressive effects of proteins on transformed cells in vitro. Our flow cytometry team has experience monitoring the proliferation and viability of stimulated T- cells or T- cells in mixed lymphocyte reactions and co-cultures using CFSE staining or other cell tracking reagents.

Labcorp has the ability to stain cultured 2D or 3D cells and acquire fluorescent images using the Cytation 3 Imaging Plate Reader. 画像が撮影されると、特殊ソフトウェアを使って核、生細胞と死細胞、染色細胞（陽性 / 陰性別）の数を計上し、サイズ、真円度、面積、周囲、各色の強度を測定します。

ホタル（P. pyralis、フォティヌス・ピラリス）のルシフェラーゼは、D-ルシフェリンがあると生物発光を起こす酵素です。ルシフェラーゼ 2 (luc2) 遺伝子の DNA を、レンチウイルス形質導入システムを用いて、ピューロマイシン耐性遺伝子とともに、細胞のゲノム中に組み込みます。細胞を培養している間にピューロマイシンを培地に加え、ルシフェラーゼ遺伝子が組み込まれた細胞のみを選択できるようにします。 Once the luc-enabled cells are injected into a mouse or rat and D-luciferin is administered, the light produced can be detected with Labcorp's IVIS® Spectrum in vivo imaging system. The in vitro services team has experience performing every step of the process from stably virally transducing cells, to selecting the transduced cells, to measuring the light output of the cells.

細胞毒性 / 増殖アッセイは、化合物ライブラリの細胞毒性スクリーニングで広く使用されています。 If you are interested in developing a therapeutic agent that targets rapidly dividing cancer cells, we can test the potency of your cytotoxic compounds.当社では、医薬品への投資に先立って、初回のハイスループットスクリーニングで、望ましくない細胞毒性効果を持つ可能性の高い「ヒット」を選別することも可能です。 Labcorp can measure the toxicity of your compound in your choice of over nearly 400 cell lines that we have availablestock in our repository. We provide graphs and IC₅₀ values for each cell line and compound.